1.The concentration is verified by optical density spectrophotometry.
2.This preparation is free of DNase and RNase contamination as determined by by agarose gel.
3. HPLC (column C18; detection UV at 253; mobile phase: A=TEAA0.1M, pH 7.0; B=60% CH3CN/A)
4. PCR with Pfu and Taq DNA Polymerases;Production of 1000bp PCR fragment
from 2ng of genomic DNA
5. Nickase is none detected by agarose gel
Storage Recommendations: Always aviod multiple freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.
Long Term (Infrequent use; 1-2 times per month): -70℃
Daily/Weekly use: -20℃
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